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Control of the phosphorylation of the astrocyte marker glial fibrillary acidic protein (GFAP) in the immature rat hippocampus by glutamate and calcium ions: possible key factor in astrocytic plasticity BJMBR
The present review describes recent research on the regulation by glutamate and Ca2+ of the phosphorylation state of the intermediate filament protein of the astrocytic cytoskeleton, glial fibrillary acidic protein (GFAP), in immature hippocampal slices. The results of this research are discussed against a background of modern knowledge of the functional importance of astrocytes in the brain and of the structure and dynamic properties of intermediate filament proteins. Astrocytes are now recognized as partners with neurons in many aspects of brain function with important roles in neural plasticity. Site-specific phosphorylation of intermediate filament proteins, including GFAP, has been shown to regulate the dynamic equilibrium between the polymerized and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Astrocytes; Glial fibrillary acidic protein; GFAP; Glutamate receptors; Protein phosphorylation; Calcium; Intermediate filament dynamics.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300005
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Expression of Vimentin and GFAP Protein of Cerebral Cortex and Its Impact on Corticogenesis Disorder as a Result of 2-Methoxyethanol International Journal of Morphology
Irnidayanti,Yulia; Darmanto,Win.
One of the plastic base material, widely used in the plastics industry in various countries, is a ester phthalate. These compounds will be oxidizedin the body to 2-methoxyethanol (2-ME). Effect of 2-ME on human health and the environment depends on the number, duration and frequency of exposure. 2-ME and its metabolites in the body can damage cells and tissues. The body can be exposed by 2-ME through the air, water and soil. Western blot results showed that the protein Vimentin was detectable in the control group at GD-11 to 17, meanwhile GFAP protein was detachable in the control group atGD- 12 to GD-18. After administration 2-ME, the expression of Vimentinprotein were changed, and started at GD- 12 up to GD-18. whereas the expression of GFAP protein...
Tipo: Journal article Palavras-chave: 2-methoxyethanol; Vimentin; GFAP; Cerebral cortex; Brain.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300003
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Glial fibrillary acidic protein (GFAP): modulation by growth factors and its implication in astrocyte differentiation BJMBR
Gomes,F.C.A.; Paulin,D.; Moura Neto,V..
Intermediate filament (IF) proteins constitute an extremely large multigene family of developmentally and tissue-regulated cytoskeleton proteins abundant in most vertebrate cell types. Astrocyte precursors of the CNS usually express vimentin as the major IF. Astrocyte maturation is followed by a switch between vimentin and glial fibrillary acidic protein (GFAP) expression, with the latter being recognized as an astrocyte maturation marker. Levels of GFAP are regulated under developmental and pathological conditions. Upregulation of GFAP expression is one of the main characteristics of the astrocytic reaction commonly observed after CNS lesion. In this way, studies on GFAP regulation have been shown to be useful to understand not only brain physiology but...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CNS; Astrocyte; Growth factors; Intermediate filaments; GFAP; Vimentin; GFAP gene.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000500016
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Redução da expressão astrocitária de proteína glial fibrilar ácida em cães tratados com dexametasona Ciência Rural
Bondan,Eduardo Fernandes; Zacariotti,Rogério Loesch; Martins,Maria de Fátima Monteiro; Viani,Flávio Cesar.
A proteína glial fibrilar ácida (GFAP) constitui o principal marcador dos astrócitos, as células gliais mais numerosas do tecido nervoso e que exibem receptores a diversos hormônios esteroidais, os quais exercem aparente influência sobre a expressão gênica das mesmas. O objetivo do presente estudo foi o de avaliar se a administração de dexametasona (DX) em protocolos terapêuticos para cães seria capaz de afetar a expressão astrocitária dessa proteína. Para tal, amostras da ponte e da medula espinhal torácica de cães, tratados (n=6) ou não (n=6) com DX, foram submetidas à marcação imuno-histoquímica para a GFAP e a reatividade astrocitária foi determinada por colorimetria em um sistema computacional de análise de imagens. Diferença estatisticamente...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Astrócitos; Cães; Corticoide; Dexametasona; GFAP; Imuno-histoquímica.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000300018
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Thyroid hormone regulates protein expression in C6 glioma cells BJMBR
Trentin,A.G.; Alvarez-Silva,M..
Thyroid hormone (T3) is essential to normal brain development. Previously, we have shown that T3 induces cerebellar astrocyte proliferation. This effect is accompanied by alteration in glial fibrillary acidic protein (GFAP) and fibronectin organization. In the present study, we report that the C6 glioma cell line, which expresses GFAP and is classified as an undifferentiated astrocytic cell type, is a target for T3 action. The C6 monolayers were treated with 50 nM T3 for 3 days, after which the cells were maintained for 2 days without medium changes. In C6 cells, T3 induced the expression of proteins of 107, 73 and 62 kDa. The hormone also up-regulated protein bands of 100 (+50%), 37 (+50%) and 25.5 kDa (+50%) and down-regulated proteins of 94 (-100%),...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Thyroid hormone; Glioma; Protein synthesis; GFAP; Vimentin; Differentiation.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000008
Registros recuperados: 5
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